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GrainGenes Probe Report: WMC99

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC99  [ Marker Report ]
Locus	Xwmc99 Xwmc99-5B wmc99 wmc99-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7c3 Donor: Somers, Agriculture Canada Trimmed to insert at 5' end. 3' end not located.(GT)13 160 to 185. (2 loci? 1 polymorph)
Type	PCR SSR
PCR primers	Forward ATTAcAATTgcTTcAgTgAgTg at 51 length 22 bases, Tm 56 Reverse TcATgATcATTgTTATAAcggT at 187 length 22 bases, Tm 56
SSR size	158 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC99
Source Species	Triticum aestivum