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GrainGenes Probe Report: WMC101

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC101
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f3 Donor: Somers, Agriculture Canada Trimmed to insert at both ends (Rsa1 at 5' end). (CA)14 38 to 65. Settings for these primers lax. Main product wrong size (300 bases) - faint bands at right size may be polymorphic.
Type	PCR SSR
PCR primers	Forward TAgTcgccATgcAATgTTcT at 8 length 20 bases, Tm 61 Reverse gAAgTgggAAggTcgATAAA at 127 length 20 bases, Tm 58
SSR size	139 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC101
Source Species	Triticum aestivum