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GrainGenes Probe Report: WMC107

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC107  [ Marker Report ]
Locus	Xwmc107
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7g6 Donor: Somers, Agriculture Canada Trimmed to insert at both ends (Rsa1 at 3'). (GT)15 82 to 111. Polymorphism is too complex to realistically score. Duplicate of 3a11(1). Bases 1 to 83 show homology to 24f1 bases 69 to 151 (complement) (83 in longest run, maxScore = 108), true duplicate, 7g6 has priority.
Type	PCR SSR
PCR primers	Forward gAATTcAggcccTTcTcggA at 12 length 20 bases, Tm 65 Reverse cATTgAAccTcgcATAAcgg at 186 length 20 bases, Tm 63
SSR size	194 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC107
Source Species	Triticum aestivum