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GrainGenes Probe Report: WMC108

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC108
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8c7 Donor: Flore, Verneuil AGR158. Trimmed to Rsa 1 sites at 5' and 3' ends(CT)13 51 to 76. Bases 50-149 (ie. 3' side of microsatellite) match part of 3G11.
Type	PCR SSR
PCR primers	Forward TcgAAgAgTAgAgTcgcTcATA at 9 length 22 bases, Tm 59 Reverse AcATAcgTgTcTgcTTAAgTgg at 106 length 22 bases, Tm 59
SSR size	119 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC108
Source Species	Triticum aestivum