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GrainGenes Probe Report: WMC117

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC117
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8g11 Donor: Flore, Verneuil AGR194 No overlap between forward and reverse sequences (weak homology in region of break). Trimmed to insert at 3' end. (GA)12 186 to 209, (GA)9 311 to 328, (GT)9 160 to 177, (GT)9 281 to 298, (GT)16 364 to 395, (GT)8 398 to 413. Bases 430-end match 2e11 (ie 3'side of mcrosatellite). Reverse sequence matches 11D5.
Type	PCR SSR
PCR primers	Forward ATgcTccTccTAgAgcATgTgT at 101 length 22 bases, Tm 62 Reverse TcgAgATgTcgATTcgTTTTcc at 480 length 22 bases, Tm 65
SSR size	401? bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC117
Source Species	Triticum aestivum