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GrainGenes Probe Report: WMC118

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC118  [ Marker Report ]
Locus	Xwmc118 Xwmc118-5B wmc118
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8h11 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 144 bases located on 5B AGR195. Double insert joined by linkers at 188-205. Small interrupted microsatellite (GT)2(GA)7 in first insert. Trimmed off Puc sequence at 3' 20.8.97 Bases 116 to 187 show homology to 20e10 bases 123 to 194 (72 in longest run, maxScore = 63).
Type	PCR SSR
PCR primers	Forward AgAATTAgcccTTgAgTTggTc at 36 length 22 bases, Tm 60 Reverse cTcccATcgcTAAAgATggTAT at 158 length 22 bases, Tm 61
SSR size	144 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC118
Source Species	Triticum aestivum