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GrainGenes Probe Report: WMC120

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC120  [ Marker Report ]
Locus	Xwmc120-1A Xwmc120 wmc120 wmc120-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8d12 Donor: Flore, Verneuil AGR199. Matches 8c12. Multiple inserts (2) joined at 314 to 331 by linkers. Trimmed to insert at 5' end and Rsa1 at 3' end. (CA)8 379 to 394, (GA)14 228 to 255, (GT)8 130 to 145. Primers designed to second insert. (2 loci - both polymorphic?)
Type	PCR SSR
PCR primers	Forward ggAgATgAgAAgggggTcAggA at 344 length 22 bases, Tm 68 Reverse ccAggAgAccAggTTgcAgAAg at 483 length 22 bases, Tm 67
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC120
Source Species	Triticum aestivum