Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC126

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC126  [ Marker Report ]
Locus	Xwmc126-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7b9 Donor: Blake, Montana 7B9 Microsatellite : [GT]~60 [AC]~30 COMBINATION. Size of microsatellite only approximate. (CA)28 257 to 312, (GT)60 136 to 255.
Type	PCR SSR
PCR primers	Forward TTgTcAcAAAAgggTcgTAAAT at 48 length 22 bases, Tm 60 Reverse ATcTcgAgATgTcATcgAAAAT at 362 length 59 bases, Tm 59
SSR size	336 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (nulls for 188 base allele).
Sequence	WMC126
Source Species	Triticum aestivum