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GrainGenes Probe Report: WMC133

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC133
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7b12 Donor: Blake, Montana Microsatellite : [AT]2 AC [TC]4 [AC]9 CAG [AC][AC]9. Tom Primer 1 : TCTCG ATCTC ATCGA GAAGG (50%). Tom Primer 2 : GCTCA AGTCA CCGCG TCAAC (60%). EXPECTED PCR PRODUCT: 152 nts.(CA)9 55 to 72, (CA)9 120 to 137.
Type	PCR SSR
PCR primers	Forward cATcTcgATcTcATcgAgAAgg at 16 length 22 bases, Tm 63 Reverse AccgcgTcAAcTAcTTAAgccA at 139 length 22 bases, Tm 65
SSR size	145 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product.
Sequence	WMC133
Source Species	Triticum aestivum