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GrainGenes Probe Report: WMC144

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC144  [ Marker Report ]
Locus	Xwmc144-2D wmc144
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5g10 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 144 bases located on 2D (CA)14 69 to 96. Bases 70-247 (complement) ie 3' side of microsatellite match 1b4, 5f2.
Type	PCR SSR
PCR primers	Forward ggAcAccAATccAAcATgAAcA at 7 length 22 bases, Tm 65 Reverse AAggATAgTTgggTggTgcTgA at 128 length 22 bases, Tm 65
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC144
Source Species	Triticum aestivum