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GrainGenes Probe Report: WMC157

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC157  [ Marker Report ]
Locus	Xwmc157-7D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11c5 Donor: Rogers, Monsanto AGR204.35.SEQ. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)29 212 to 269. Redesign primers? Bases 115-229 (ie 5' side of microsatellite) match part of 5g9. Bases 189 to 263 show homology to 24c1 bases 82 to 156 (75 in longest run, maxScore = 82), duplicate, 11c5 has precedence.
Type	PCR SSR
PCR primers	Forward cTTgATccAAgTggTTcTTTcc at 183 length 22 bases, Tm 62 Reverse TccAAATgTTTgcgAAAccTgA at 270 length 22 bases, Tm 66
SSR size	109 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, product;
Sequence	WMC157
Source Species	Triticum aestivum