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GrainGenes Probe Report: WMC173

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC173  [ Marker Report ]
Locus	Xwmc173-4A.1 [ Show all 12 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9f3.2 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 263-279 bases located on 4A Trimmed to Rsa1 at both ends. Multiple inserts (2) joined at 382-399 by linkers. (CA)13 705 to 730, (GA)8 121 to 136. Primers shown are for second insert.
Type	PCR SSR
PCR primers	Forward TgcAgTTgcggATccTTgA at 532 length 19 bases, Tm 66 Reverse TAAccAAgcAgcAcgTATT at 786 length 19 bases, Tm 56
SSR size	273 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC173
Source Species	Triticum aestivum