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GrainGenes Probe Report: WMC174

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC174
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h3 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at 5' and to insert at 3' end. (GA)9 173 to 190. Difficult to design primers, reverse primer has potentially 2 binding sites subsequent serching showed is member of 1g1 etc.
Type	PCR SSR
PCR primers	Forward AggAAcAAATgcTccgcc at 111 length 18 bases, Tm 63 Reverse gcAcgcATgcAcgcAccc at 214 length 18 bases, Tm 73
SSR size	121 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, smear;
Sequence	WMC174
Source Species	Triticum aestivum