Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC176

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC176
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9d4 Donor: Bernard, Clermont-Ferrand Trimmed to insert at both ends. (GA)8 122 to 137, many small microsatellite motifs. Bases 207-440 complement (ie. 3' side of microsatellite) match part of 6f5. Bases 379 to 396 show homology to 12d8.1 bases 43 to 60 (complement) (18 in longest run, maxScore = 28). Bases 374 to 438 show homology to 21h5 bases 8 to 72 (complement) (65 in longest run, maxScore = 83). Bases 243 to 288 show homology to 25d8 bases 49 to 94 (46 in longest run, maxScore = 105). Duplicate with 25d8, 9d4 has priority.
Type	PCR SSR
PCR primers	Forward TggATgAAgggTggTAggAAcA at 28 length 22 bases, Tm 65 Reverse TAAATTgcATccgccATAgAgg at 328 length 22 bases, Tm 65
SSR size	322 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC176
Source Species	Triticum aestivum