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GrainGenes Probe Report: WMC187

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC187
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4a8 Donor: Morell, Canberra Trimmed to insert at 5' end and to Rsa1 at 3' end (1 N in linker). (GT)10 200 to 219, (GT)14 234 to 261, (GT)8 386 to 401.
Type	PCR SSR
PCR primers	Forward cTTcgcAgcTgTcTcATTcgcA at 99 length 22 bases, Tm 70 Reverse ATATccgcgAgcgcAgAAggTc at 364 length 22 bases, Tm 70
SSR size	287 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC187
Source Species	Triticum aestivum