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GrainGenes Probe Report: WMC193

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC193  [ Marker Report ]
Locus	Xwmc193-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4f9 Donor: Morell, Canberra Trimmed to Rsa1 at both ends. Multiple inserts (2) in this clone joined 330-347 by linkers. (CA)30 382 to 441. Primers are for second insert. Bases 217-327 (ie 3' die poly C microsatelllite) match part of 3D9. Bases 264 to 300 show homology to 17a10 bases 90 to 126 (complement) (37 in longest run, maxScore = 45). Bases 224 to 300 show homology to 22d8 bases 40 to 116 (complement) (77 in longest run, maxScore = 68), true duplicate, 4f9 has priority.
Type	PCR SSR
PCR primers	Forward cgTggAcTAAcAAgTcATTT at 348 length 20 bases, Tm 55 Reverse gTcATcATTTTccccggcAT at 444 length 20 bases, Tm 66
SSR size	116 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC193
Source Species	Triticum aestivum