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GrainGenes Probe Report: WMC196

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC196
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4d10 Donor: Morell, Canberra Trimmed to Rsa1 at both ends. (CA)14 75 to 102. Bases 86-182 (ie. 3' side of microsatellite) match 4b10. Bases 145 to 182 show homology to 19c3 bases 27 to 64 (complement) (38 in longest run, maxScore = 53). Bases 111 to 130 show homology to 10b9 bases 55 to 74 (complement) (20 in longest run, maxScore = 36).
Type	PCR SSR
PCR primers	Forward ccgTAcggAgTcAggTcgATgT at 21 length 22 bases, Tm 67 Reverse gcTTcgcAcTTgcAcgATTTTc at 133 length 22 bases, Tm 68
SSR size	134 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC196
Source Species	Triticum aestivum