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GrainGenes Probe Report: WMC197

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC197
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c11 Donor: Morell, Canberra Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases131-380 (ie. 3' side of microsatellite) matches 6d5 , highly related to 2e10, 1g12. . (GA)9 149 to 166, (GT)8 133 to 148.
Type	PCR SSR
PCR primers	Forward ccTAATTAAAAgcTgTcAcgAg at 63 length 22 bases, Tm 58 Reverse gcAATgcATATggcTgAAAgAT at 206 length 22 bases, Tm 63
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC197
Source Species	Triticum aestivum