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GrainGenes Probe Report: WMC198

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC198  [ Marker Report ]
Locus	Xwmc198 Yr32
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4d11 Donor: Morell, Canberra Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)27 67 to 120.
Type	PCR SSR
PCR primers	Forward cAcgcTgccATcAcTTTTAc at 27 length 20 bases, Tm 61 Reverse TTgAAgTggTcATTgTTgcT at 166 length 20 bases, Tm 59
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC198
Source Species	Triticum aestivum