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GrainGenes Probe Report: WMC199

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC199
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4a12 Donor: Morell, Canberra Trimmed to insert at 5' end (removing mostly Ns), trimmed to Rsa1 at 3' end. Compound microsatellite (NA)10(CA)3(GA)(CA)2(TA)(CA)6.
Type	PCR SSR
PCR primers	Forward cAcAcTcATAgTTATTAcgccT at 297 length 22 bases, Tm 55 Reverse cTATTTAcTcAcTgAcTgggAA at 378 length 22 bases, Tm 55
SSR size	103 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC199
Source Species	Triticum aestivum