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GrainGenes Probe Report: WMC200

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC200
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4d12 Donor: Morell, Canberra Trimmed to Rsa1 at the 5' end, and to insert at 3' end. (GT)8 56 to 71, (GT)10 97 to 116.
Type	PCR SSR
PCR primers	Forward TAcgTgAgAgAgTcccTAAc at 2 length 20 bases, Tm 54 Reverse cTccTcAcgAggcTTccATA at 202 length 20 bases, Tm 62
SSR size	220 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC200
Source Species	Triticum aestivum