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GrainGenes Probe Report: WMC205

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC205
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3d1 Donor: Jack, Plant Breeding International No trimming done to insert. Bases 50-161 (complement) (ie. 3' side of microsatellite) match 6f11(2) and part of 5h4. (GA)12 51 to 74.
Type	PCR SSR
PCR primers	Forward gcTTAgcAATAAgcTTggcAgg at 15 length 22 bases, Tm 64 Reverse ccgTTgTcAAAgATgcATgTgT at 166 length 22 bases, Tm 65
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide. (several loci).
Sequence	WMC205
Source Species	Triticum aestivum