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GrainGenes Probe Report: WMC207

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC207
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3a3 Donor: Jack, Plant Breeding International No trimming done to insert. Bases 151-284 (complement) (ie. 3' side of microsatellite) match part of 6F4 (the reverse primer shown is not included in this region of homology). (CT)32 57 to 120. The primers have very high Tms. If they give a smear try decreasing the length.
Type	PCR SSR
PCR primers	Forward cTcccTccTccccTgcAAcAcc at 35 length 22 bases, Tm 72 Reverse ggcAgggcTTcgccgAgcTTcT at 123 length 22 bases, Tm 75
SSR size	110 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product ( beware primer dimer).
Sequence	WMC207
Source Species	Triticum aestivum