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GrainGenes Probe Report: WMC212

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC212
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3h5 Donor: Jack, Plant Breeding International No trimming done to insert. (GT)11 158 to 179. A stuggle to get these primers - note very low Tms.
Type	PCR SSR
PCR primers	Forward AATATTAgcAgAATAgAcgg at 135 length 20 bases, Tm 51 Reverse TgTATgcTcTAgcTAgATgT at 235 length 20 bases, Tm 49
SSR size	120 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC212
Source Species	Triticum aestivum