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GrainGenes Probe Report: WMC226

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC226
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g10.2 Donor: Benoist AGR205.31. Trimmed to Rsa1 at both ends. Multiple inserts in this clone joined at 261-278 by linkers. (CA)13 109 to 134, (GT)25 405 to 454. Primers are from second insert.
Type	PCR SSR
PCR primers	Forward gATcgcccAcTAgggTATAAATgA at 306 length 24 bases, Tm 64 Reverse gTgTTATcgTgAggAgAggcAAAA at 524 length 24 bases, Tm 65
SSR size	242 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC226
Source Species	Triticum aestivum