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GrainGenes Probe Report: WMC227

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC227
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11d11 Donor: Benoist AGR205.36. Trimmed to Rsa1 at 5' end and to insert at 3' end. Similar to, but not identical to 11e11. Bases 154-280 are from the 1g1 family - caution. (CA)12 48 to 71. Bases 195 to 226 show homology to 14c9 bases 48 to 79 (complement) (32 in longest run, maxScore = 61).
Type	PCR SSR
PCR primers	Forward ATTTccTgAAAcAcAccccg at 12 length 20 bases, Tm 64 Reverse cgTTTggcAggcgTcATcTA at 241 length 20 bases, Tm 66
SSR size	249 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC227
Source Species	Triticum aestivum