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GrainGenes Probe Report: WMC252

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC252
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g1 Donor: Gupta, Meerut No trimming done to sequence. (CA)14 128 to 155.
Type	PCR SSR
PCR primers	Forward TcAcTATgcTcAcTcTTcggcT at 93 length 22 bases, Tm 63 Reverse cTccTcgTgTTccTgATATgcT at 179 length 22 bases, Tm 62
SSR size	108 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC252
Source Species	Triticum aestivum