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GrainGenes Probe Report: WMC261

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC261  [ Marker Report ]
Locus	Xwmc261-2A [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4e5 Donor: Gupta, Meerut Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)12 68 to 91.
Type	PCR SSR
PCR primers	Forward gATgTgcATgTgAATcTcAAAAgTA at 37 length 25 bases, Tm 62 Reverse AAAgAgggTcAcAgAATAAccTAAA at 122 length 25 bases, Tm 60
SSR size	110 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC261
Source Species	Triticum aestivum