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GrainGenes Probe Report: WMC262

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC262  [ Marker Report ]
Locus	Xwmc262-4A Xwmc262 wmc262 wmc262-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g5 Donor: Gupta, Meerut Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)29 86 to 143.
Type	PCR SSR
PCR primers	Forward gcTTTAAcAAAgATccAAgTggcAT at 9 length 25 bases, Tm 65 Reverse gTAAAcATccAAAcAAAgTcgAAcg at 182 length 25 bases, Tm 64
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC262
Source Species	Triticum aestivum