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GrainGenes Probe Report: WMC264

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC264  [ Marker Report ]
Locus
Xwmc264-3A
[ Show all 9 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4c6
Donor: Gupta, Meerut
No trimming done to sequence. (GA)16 81 to 112.
Type
PCR
SSR
PCR primers
Forward cTccATcTATTgAgcgAAggTT at 55 length 22 bases, Tm 61
Reverse cAAgATgAAgcTcATgcAAgTg at 166 length 22 bases, Tm 63
SSR size
133 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC264
Source Species
Triticum aestivum