Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC274

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC274  [ Marker Report ]
Locus	Xwmc274-3B wmc274 wmc274-3B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3h8 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 3B No trimming done to sequence. (GAAAA)9 microsatellite 145-187.
Type	PCR SSR
PCR primers	Forward AAgcAAgcAgcAAAAcTATcAA at 92 length 22 bases, Tm 61 Reverse gAATgAATgAATgAATcgAggc at 209 length 22 bases, Tm 62
SSR size	139 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC274
Source Species	Triticum aestivum