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GrainGenes Probe Report: WMC284

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC284
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13e1 Donor: Jager-Gussen, Saaten Union Trimmed to insert at 3' end, 5' end not located. (GT)8 552 to 567. Bases 81 to 90 show homology to 12d7 bases 32 to 41 (complement) (10 in longest run, maxScore = 29).
Type	PCR SSR
PCR primers	Forward gAAgggTggTgggAAcAAAT at 478 length 20 bases, Tm 63 Reverse acAcAcTAAgAcTccATcTATc at 580 length 22 bases, Tm 52
SSR size	124 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC284
Source Species	Triticum aestivum