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GrainGenes Probe Report: WMC290

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC290
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13e2 Donor: Jager-Gussen, Saaten Union Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 10-218 (ie. 5' side of microsatellite) match part of 5c7 - forward primer has 5 mismatches to 5C7. (GT)9 201 to 218, (GT)12 221 to 244.
Type	PCR SSR
PCR primers	Forward gTgccATcATcAcTgcAAcTgT at 77 length 22 bases, Tm 65 Reverse cTggTgcgAAgTcTggcTATcA at 272 length 22 bases, Tm 67
SSR size	217 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide (several loci), beware primer dimer.
Sequence	WMC290
Source Species	Triticum aestivum