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GrainGenes Probe Report: WMC292

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC292
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13c3 Donor: Jager-Gussen, Saaten Union Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 96 -242 (complement) (ie. 3' side of microsatellite match part of 6d12. Reverse primer has 2 mismatches to 13c3. (GA)18 86 to 121, (GA)9 124 to 141.
Type	PCR SSR
PCR primers	Forward gAggATAcggcTggATcTgTgA at 51 length 22 bases, Tm 65 Reverse TcTcgTccTcTccAgATcTAAA at 160 length 22 bases, Tm 60
SSR size	131 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC292
Source Species	Triticum aestivum