Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC298

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC298
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f6 Donor: Jager-Gussen, Saaten Union Trimmed to insert at both ends. (GA)16 271 to 302, (GT)18 235 to 270.
Type	PCR SSR
PCR primers	Forward cTcTTgcATAcAAATgccAggA at 177 length 22 bases, Tm 64 Reverse ctAAAcAcTTcTcTgccTAggAcA at 330 length 24 bases, Tm 62
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product. Primer dimer - redesign primers.
Sequence	WMC298
Source Species	Triticum aestivum