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GrainGenes Probe Report: WMC299

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC299
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13h6 Donor: Jager-Gussen, Saaten Union Trimmed to Rsa1 at both ends. Apart from a few indels this sequence (complement) is a duplicate of 9F3.1 - forward primer has no match to 9f3.1, and reverse primer has 2 mismatches including antepenultimate base. (CT)8 187 to 202. Bases 10 to 36 show homology to 15g2 bases 471 to 497 (complement) (27 in longest run, maxScore = 113).
Type	PCR SSR
PCR primers	Forward gtacTccATgAAcATgTTgAAAg at 1 length 23 bases, Tm 58 Reverse gggAcTAAcAAggTTTgcgT at 239 length 20 bases, Tm 61
SSR size	258 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC299
Source Species	Triticum aestivum