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GrainGenes Probe Report: WMC300

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC300
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8A1 Donor: Rouse, Novartis Contains (GT)19(GA)13 repeat. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 189-239(ie. 3' side of microsatellite) match part of 1f11 (1 mismatch in reverse primer). (GA)13 180 to 205, (GT)19 142 to 179.
Type	PCR SSR
PCR primers	Forward cAcgAAgcgAcgTAgggTTATT at 22 length 22 bases, Tm 64 Reverse cAgAgcAccTTTTgTgTTgAgA at 208 length 22 bases, Tm 62
SSR size	208 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; complex.
Sequence	WMC300
Source Species	Triticum aestivum