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GrainGenes Probe Report: WMC302

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC302
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8e1 Donor: Rouse, Novartis Contains (AC)18 and (AC)11 imp. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)17 148 to 181. Bases 170-268 (complement) ie 3' side of microsatellite match part of 8C12. Reverse primer is outside this region. 8F2 is a duplicate of this.
Type	PCR SSR
PCR primers	Forward cAAcccTgTgTAATTgTAAccg at 76 length 22 bases, Tm 61 Reverse ggTAggcAcAgAggcAcAAgTA at 279 length 22 bases, Tm 63
SSR size	225 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, many products, some polymorphic.
Sequence	WMC302
Source Species	Triticum aestivum