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GrainGenes Probe Report: WMC305

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC305
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8h4 Donor: Rouse, Novartis Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)21 87 to 128.
Type	PCR SSR
PCR primers	Forward AcccTAAgAggTggTTcTgTTg at 43 length 22 bases, Tm 61 Reverse gAAccAgcccTAccAcAcATAA at 189 length 22 bases, Tm 63
SSR size	168 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product. Not polymorphic on tested varieties;
Sequence	WMC305
Source Species	Triticum aestivum