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GrainGenes Probe Report: WMC307

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC307  [ Marker Report ]
Locus	Xwmc307-3B wmc307-3B wmc307
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8c5 Donor: Rouse, Novartis Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 146 bases located on 3B Contains (GT)8(GA)13. Trimed to Rsa1 at 5' end and to insert at 3 ' end. (GA)13 171 to 196, (GT)8 155 to 170.
Type	PCR SSR
PCR primers	Forward gTTTgAAgAccAAgcTccTccT at 81 length 22 bases, Tm 63 Reverse AccATAAccTcTcAAgAAcccA at 204 length 22 bases, Tm 62
SSR size	145 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC307
Source Species	Triticum aestivum