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GrainGenes Probe Report: WMC309

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC309
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a1 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 118 bases located on 3D, 179 bases located on 3D Trimmed to insert at 5' end and to Rsa1 at 3' end. (GGC)7 120 to 140.
Type	PCR SSR
PCR primers	Forward gATgcTTgggcAgTTTTAccgT at 56 length 22 bases, Tm 66 Reverse gAAgAAgTgcATAgAgcAgggA at 211 length 22 bases, Tm 63
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC309
Source Species	Triticum aestivum