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GrainGenes Probe Report: WMC313

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC313  [ Marker Report ]
Locus	Xwmc313-4A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr60 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14f2 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 198 bases located on 4A Trimmed to Rsa1 at both ends. (CA)18 166 to 201.
Type	PCR SSR
PCR primers	Forward gcAgTcTAATTATcTgcTggcg at 78 length 22 bases, Tm 63 Reverse gggTccTTgTcTAcTcATgTcT at 253 length 22 bases, Tm 59
SSR size	197 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC313
Source Gene	Yr60 (Triticum)
Source Species	Triticum aestivum