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GrainGenes Probe Report: WMC316

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC316
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14h3 Donor: Hjortsholm, Sejet Trimmed to insert at 5' end and to insert at 3' end. Bases 155-328 match part of 6h4 ( a member of 1g1 family). Reverse primer has 2 mismatches to 6h4. (CA)8 156 to 171.
Type	PCR SSR
PCR primers	Forward ATgAAcAcAgccAAgATgAAgg at 134 length 22 bases, Tm 63 Reverse AgcgTgTgTgTgAgATggAgTc at 176 length 22 bases, Tm 65
SSR size	64 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC316
Source Species	Triticum aestivum