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GrainGenes Probe Report: WMC320

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC320  [ Marker Report ]
Locus	Xwmc320 wmc320
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a6 Donor: Hjortsholm, Sejet Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)12 24 to 47.
Type	PCR SSR
PCR primers	Forward cATgcTTTcAAgAccTAcgAcA at 4 length 22 bases, Tm 62 Reverse gcAAAcTTgcTcTgcTTgAcTc at 78 length 22 bases, Tm 63
SSR size	96 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC320
Source Species	Triticum aestivum