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GrainGenes Probe Report: WMC323

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC323  [ Marker Report ]
Locus	Xwmc323-7B Xwmc323 wmc323 wmc323-7B Xwmc323-7A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17e7 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 160 bases located on 7B Trimmed to insert at both ends. Bases 273 to 293 show homology to 9h4 bases 81 to 101 (21 in longest run, maxScore = 21) = mostly tetranucleotide repeat. (CA)11 253 to 274, (CA)11 297 to 318, (CT)11 231 to 252.
Type	PCR SSR
PCR primers	Forward AcATgATTgTggAggATgAggg at 180 length 22 bases, Tm 65 Reverse TcAAgAggcAgAcATgTgTTcg at 319 length 22 bases, Tm 65
SSR size	161 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC323
Source Species	Triticum aestivum