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GrainGenes Probe Report: WMC336

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC336  [ Marker Report ]
Locus	Xwmc336a [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a4 Donor: Weibull, Svaloff Weibull Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 112 bases located on 1D, 129 bases located on 1A Trimmed to insert at 5' end and to Rsa1 at 3' end. (GCC)7 165 to 185.
Type	PCR SSR
PCR primers	Forward gTcTTAccccgcgATcTgc at 102 length 19 bases, Tm 65 Reverse gcggccTgAgcTTcTTgAg at 196 length 19 bases, Tm 65
SSR size	113 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC336
Source Species	Triticum aestivum