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GrainGenes Probe Report: WMC347

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC347
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19c1 Donor: Pioneer No overlap between forward and reverse sequences. Trimmed to insert at both ends. Bases 59 to 80 (complement) show homology to 7b1 bases 51 to 72 (22 in longest run, maxScore = 19), zone is 58-123. Primers are not in this area. (GT)9 806 to 823, plus small GT runs. Bases 26 to 56 show homology to 10h7.2 bases 88 to 118 (31 in longest run, maxScore = 39). Bases 37 to 80 show homology to 21b3 bases 166 to 209 (complement) (44 in longest run, maxScore = 35). Bases 12 to 43 show homology to 24f9 bases 2 to 33 (32 in longest run, maxScore = 28).
Type	PCR SSR
PCR primers	Forward gATTTTTAccAAAATcATTAggggA at 719 length 25 bases, Tm 62 Reverse cATggTggTAgTTTTATTTTgAAgA at 886 length 25 bases, Tm 60
SSR size	192 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product.
Sequence	WMC347
Source Species	Triticum aestivum