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GrainGenes Probe Report: WMC348

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC348
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19d1 Donor: Pioneer Trimmed to insert at both ends. Bases 273 to 309 show homology to 6b12 bases 55 to 91 (37 in longest run, maxScore = 90), zone is 231-417. (CT)12 29 to 52, Bases 148 to 215 show homology to 22f10 bases 396 to 463 (68 in longest run, maxScore = 119).
Type	PCR SSR
PCR primers	Forward AccccgAcTAccgTcTcc at 12 length 18 bases, Tm 62 Reverse AATAcgAgTcggggTgcg at 135 length 18 bases, Tm 64
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC348
Source Species	Triticum aestivum