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GrainGenes Probe Report: WMC349

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC349  [ Marker Report ]
Locus	Xwmc349-4B Xwmc349 wmc349
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19a2 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 102 bases located on 4B Trimmed to insert at both ends. (GT)22 123 to 166.
Type	PCR SSR
PCR primers	Forward AcAcAcAcTcgATcgcAc at 86 length 18 bases, Tm 59 Reverse gcAgTTgATcATcAAAAcAcA at 162 length 21 bases, Tm 60
SSR size	94 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC349
Source Species	Triticum aestivum