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GrainGenes Probe Report: WMC350

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC350  [ Marker Report ]
Locus	Xwmc350-5A Xwmc350
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19b2 Donor: Pioneer Trimmed to insert at both ends. Bases 258 to 307 show homology to 1C2 bases 305 to 354 (50 in longest run, maxScore = 33), zone is 250-327. Reverse primer has a mismatch to 1C2. (CT)15 258 to 287.
Type	PCR SSR
PCR primers	Forward gTcAgTccATcgAAgccTcc at 180 length 20 bases, Tm 64 Reverse TccgAgAAAgAgcgAggTcA at 302 length 20 bases, Tm 65
SSR size	142 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC350
Source Species	Triticum aestivum